Terminator

Part:BBa_K1968014:Design

Designed by: Alejandra Adriana Schiavon Osorio   Group: iGEM16_Edinburgh_OG   (2016-10-12)


Tcyc Phytobrick: cytochrome C gene transcriptional terminator from Saccharomyces cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Phytrobrick format and MoClo compatible (Overhangs D and F)


Source

Genomic Sequence. The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection and was then assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing

References